HTCL

 

High Throughput Microbial Cultivation Lab (HTCL)

One of our major activities is the culturing of new microbial species. Our earlier research on applications of molecular biology to microbial ecology helped establish the broad and fundamental observation that a majority of the microorganisms in natural ecosystems have not been cultured in scientific laboratories.

Bacteria that have never been cultured are among the most numerous organisms on our planet. Understanding their activities is critical to the development of local and global geochemical models, as well as socially relevant issues such as bioremediation. They are also a major reservoir of natural diversity that is yielding new enzymes and small molecules for the chemical industry, agriculture, and medicine.

To address this problem, we devised and built a new experimental facility at OSU for studying high throughput microbial cultivation methods. Our facility uses robotics, flow cytometry and cell arraying techniques to culture and identify cells at the very low growth rates and nutrient concentrations that are typical of natural ecosystems.

We seek to culture these organisms because their complete genome sequences may offer one of the best sources of insight into their activities in nature, and because cell cultures provide opportunities to test hypotheses about cellular functions that arise from genome analysis.

 

Strain ID Genus/Species Location Collected Phylum/Class
HTCC2155 Lentisphaera araneosa  Coastal Pacific Ocean, Newport, Oregon  Lentisphaerae
HTCC2143 Not yet validly described  Coastal Pacific Ocean, Newport Oregon  Gammaproteobacteria
HTCC2207 Not yet validly described  Coastal Pacific Ocean, Newport, Oregon  Gammaproteobacteria
HTCC2503 Parvularcula bermudensis  Sargasso Sea, Atlanic Ocean, BATS  Alphaproteobacteria
HTCC2506 Fulvimarina pelagi  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2559 Croceibacter atlanticus  Sargasso Sea, Atlantic Ocean, BATS  Bacteriodetes
HTCC2501 Robiginitalea biformata  Sargasso Sea, Atlantic Ocean, BATS  Bacteriodetes
HTCC2516 Oceanicola granulosus  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobaceria
HTCC2597 Oceanicola bastensis  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2255 Not yet validly described, OM42 clade  Coastal Pacific Ocean, Newport, Oregon  Alphaproteobacteria
HTCC2654 Maritimibacter alkaliphilus  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2083 Not yet validly described, novel genus level  Coastal Pacific Ocean, Newport Oregon  Alphaproteobacteria
HTCC2170 Not yet validly described, novel genus level  Coastal Pacific Ocean, Newport, Oregon  Bacteroidetes
HTCC2601 Pelagibaca bermudensis  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2649 Janibacter sp.  Sargasso Sea, Atlantic Ocean, BATS  Actinobacteria
HTCC2633 Oceanicaulis alexandrii  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2594 Erythrobacter litoralis  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC1002 Candidatus 'Pelagibacter ubique' SAR11  Coastal Pacific Ocean, Newport, Oregon  Alphaproteobacteria
HTCC1062 Candidatus 'Pelagibacter ubique' SAR11  Coastal Pacific Ocean, Newport, Oregon  Alphaproteobacteria
HTCC2080 Not yet validly described, OM60 clade  Coastal Pacific Ocean, Newport, Oregon  Gammaproteobacteria
HTCC2181 Not yet validly described, OM43 clade  Coastal Pacific Ocean, Newport, Oregon  Baetaproteobacteria
HTCC5015 Not yet validly described  Sargasso Sea, Atlantic Ocean, BATS  Gammaproteobacteria
HTCC2150 Not yet validly described, RCA clade  Coastal Pacific Ocean, Newport, Oregon  Alphaproteobacteria
HTCC7211 Candidatus 'Pelagibacter ubique' SAR11  Sargasso Sea, Atlantic Ocean, BATS  Alphaproteobacteria
HTCC2148 Not yet validly described, OM60 clade  Coastal Pacific Ocean, Newport, Oregon  Gammaproteobacteria

 

HTCL Contributions

HTCC strains used by other researchers.  Use of strains or DNA requires a Uniform Biological Material Transfer Agreement.  Upon receiving a request for materials, the OSU Office for Commercialization and Corporate Development will assist the requesting institution to negotiate the transfer.  Note that the transmittal fee is waived for academic institutions.  Alternatively, strains HTCC2155,2156,2501,2503,2506,2516,2559, and 2597 are available through the ATCC.

HTCC2649 DNA Dr. Manfred Hoefle Helmholtz Centre for Infection Res.  
HTCC2594 DNA Dr. Laurie Richardson Florida Intl. U.  
HTCC2503/2559/2506 DNA Dr. Lara K Mahal U. of Texas at Austin  
HTCC2150 Strain Dr. Meinhard Simon U. of Oldenburg  
HTCC170 DNA Dr. Rob Wolthuis Netherlands Cancer Inst.  
HTCC2594 DNA Dr. Trevor Swartz U. of California, Santa Cruz Swartz et al., 2007; Science 317:1090-3
HTCC2654 DNA Dr. Rayford Payne Georgia Inst. of Technology Payne et al., 2008; Cloning and
molecular characterization of the key
denitrase involved in 3-nitrotyrosine catabolism. ABM.
HTCC2649 DNA Dr. John Gerit U. of Illinois  
HTCC2503/2597/2633 DNA Dr. Alexandra Z Worden Monterey Bay Aquarium Res. Inst.  
HTCC2633 Strain Dr. David Green Dunstaffnage Marine Lab.  
HTCC2594/ Strains Emily Schubert Northwestern High School, Indiana  
HTCC2207 DNA Dr. Pierre Nioche L'Université Paris Descartes  
HTCC2594 Strain Dr. Dan Kunz University of North Texas  
HTCC2501 Strain Dr. Anne-Lisa Ducluzeau L'Université Paris Descartes  
HTCC2594 Strain Dr. Vincent Martin Concordia U.  
HTCC2633 Strain Dr. Yves V Brun Indiana U.  
HTCC2649 DNA Dr. Aidan Doherty U. of Sussex  
HTCC2080 DNA Dr. Michal Koblizek Inst. of Microbiology, Czech Republic  
HTCC2148 DNA Dr. Bernhard Fuchs Max Planck Inst. for Marine
Microbiology, Bremen
 
HTCC2170 DNA Dr. Adrian Hugenmatter Weizmann Inst. of Science  
HTCC2649 DNA Dr. Yogesh S Shouche National Centre for Cell Science, India  
HTCC25-3/2633 DNA Dr. Juan Cabrera-Luque Children'a National Medical Center, D.C.  
HTCC1062 DNA Dr. Alison E Murray Desert Res. Inst. Nevada  
HTCC2170 DNA Dr. Adrian Hugenmatter Weizmann Inst. of Science, Israel  
HTCC1062,1002,7211,
2207,2080,9004,5015,2155,2181
Strain Drs. Craig Carlson, Robert Morris U. of California, Santa Barbara  
HTCC2649/7211 DNA Dr. Nora Ausmees Uppsala U., Sweden  
HTCC2170 DNA Dr. Stefan Warmuth U. of Zurich  
HTCC2594 Strain Dr. James Anthony Goodwin U. of California, Santa Cruz  
HTCC2597/2506 Strains Dr. Andy Johnston U. of East Anglia, U.K. Curson et al., 2008; Env. Microbiol.
10:757-67, Corrigendum
HTCC2594 DNA Dr. Kevin Gardner U. of Texas Southwestern Med. Ctr.  
HTCC2516,2597,2654,2601,2083 Strains Dr. Matt Sullivan Massachusetts Inst. of Technology  
HTCC2080 Strain Dr. Rebecca Case Harvard Medical School  
HTCC1062 DNA Dr. Rebecca Case Harvard Medical School  
HTCC2633,2597,2516,2150
2654,2601
DNA Dr. Torsten Thomas U. of new South Wales  
HTCC2594,2501 DNA Dr. Ken Ishikawa U. of Tokyo  
HTCC1062 DNA Dr. Ranjai Kumar Panjab U.  
HTCC1062 DNA Dr. Ken Ryan Victoria U. of Wellington  
HTCC2503 Strain Dr. Arun Bhagwath National Chung Hsing U.  
HTCC1062 Strain Dr. Christina Preston Monterey Bay Aquarium Res. Inst.  
HTCC2594 Strain Dr. Arlene LM Haffa U. of Wisconsin, Oshkosh  
HTCC2155 DNA Dr. Jan Pané-Farré Ernst-Moritz-Arndt-U. of Greifswald  
HTCC1062 DNA Dr. Mishtu Dey Massachusetts Inst. of Technology  
HTCC2597 Strain Dr. John P Quinn Queen's University, Belfast  
HTCC2503,2597,2633,
2559,2501,2170
Strains Dr. Nicole King U. of California, Berkeley  
HTCC2633 Strain Dr. Lauren McDaniel U. of South Florida  
HTCC2601 DNA Dr. Mary Ann Moran U. of Georgia  
HTCC1002,2506 DNA Dr. Felix Sangari U. of Cantabria  
HTCC2649 DNA Dr. Ronan Kelly U. College Dublin  
HTCC2083 Strain Dr. Bjarne Jochimsen U. of Aarhus  
HTCC1062,7201 DNA Dr. Amy Zimmerman U. of Calfornia, Irvine  
HTCC1062 DNA Dr. Daniel Herlemann Leibniz-Institut für Ostseeforschung  
HTCC5015 DNA Dr. Tim Sampson Emory U.  
HTCC2601,2255 DNA Dr. Andy Johnston U. of East Anglia  
HTCC2601,2597,2516 DNA Dr. David Emerson Bigelow Lab. for Ocean Sciences  
HTCC2594 DNA Dr. James Goodwin U. of California, Santa Cruz  
HTCC1002 DNA Sr. Dr. Rich Roberts New England Biolabs  
HTCC2155 DNA Dr. George Fuchs U. of Freiburg  
HTCC2255 DNA Dr. Irene Wagner-Dobler Helmholtz Centre for Infection Res.  
HTCC2155 DNA Dr. Farooq Azam Scripps Inst. of Oceanography, UCSD  
HTCC1962,2255,2148,2080,2150,
2506,2170,2143,2207,2654,2597,2516
Strains Dr. Laura Steindler U. of Haifa  
HTCC1062 DNA Dr. Alexander Bochdansky Old Dominion U.  
HTCC7211 Strain Dr. Buford Price U. of California, Berkeley  
HTCC2181 DNA Dr. Yale Yuen U. of California, Merced  
HTCC1062 Strain Dr. Penny Chisholm MIT  
HTCC7211 Strain Dr. Penny Chisholm MIT  
HTCC1062 Strain Dr. Dave Kirchman U. of Delaware  
HTCC7211 Strain Dr. Dave Kirchman U. of Delaware