Dr. Michael Kent, Professor

  Office 532 Nash Hall
Phone 541-737-8652
FAX 541-737-0496
Email Michael.Kent@oregonstate.edu
Education    
Ph.D. University of California

Research InterestsFish Diseases, Parasitology, Pathogens of Zebra Fish

Courses Taught:  MB 480/580 General Microbiology; BHS 329 Mechanisms of Disease;  VMB 760 Veterinary Parasitology; Health and Colony Management of Laboratory Fish, An annual summer course at the Center for Science Entrepreneurship in Bar Harbor, Maine,  MDI Biological Laboratory.

RESEARCH 

Presently the Kent lab is focusing on two major research areas; diseases of zebrafish in research facilities and impacts of pathogens on wild fishes in Oregon. In both areas, we are focusing on the study of chronic infectious diseases. In the past decade there has been a dramatic increase in the use of zebrafish as a model in biomedical research. Underlying chronic diseases are of concern as they relate to non-protocol induced variation in laboratory fish, as they would with any laboratory animal.  Microsporidosis (caused by Pseudoloma neurophilia); mycobacteriosis (caused by various species of Mycobacterium); the nematode Pseudocapillaria tomentosa, and Mycoplasma-related intestinal cancers are all common in zebrafish facilities, and are under investigation at this time. This work is supported by NIH Office of Research Infrastructure Programs (NIH ORIP), National Institute of Allergy and Infectious Diseases (NIH NIAID), and the National Cancer Institute (NIH NCI).  Co-investigators for this research at OSU include Dr. Thomas Sharpton (Departments of Microbiology and Statistics), Dr. Justin Sanders (Department of Biomedical Sciences), and Dr. Karen Guillemin (University of Oregon).  Dr. Kent is also a Co-PI at the Zebrafish International Resource Center at the University of Oregon where he assists with health studies and their diagnostic service.  esDr. Kent was instrumental in the design of an on-line manual on zebrafish diseases for the research community and he and Dr. Robert Tanguay established the SPF (Specific Pathogen Free) zebrafish research colony at the Sinnhuber Aquatic Resource Center (OSU).  This was supported by the NIEHS Environmental Health Sciences Center and NIH ORIP.  

Wild Fishes in Oregon: for the last several years we have been investigating the role of multiple pathogen infections with prespawning Chinook salmon from the Willamette River in collaboration with Dr. Carl Schreck and Dr. James Peterson (Dept. of Fisheries and Wildlife, OSU; and USGS) and Dr. Justin Sanders through the support of the U.S. Army Corps of Engineers.  The Kent lab has also recently completed a study on the impacts of parasites on endangered sucker species in the Klamath Lake in collaboration with Dr. Doug Markle (Dept. of Fish and Wildlife, OSU) and Dr. James Peterson.  This research is funded by the U.S. Bureau of Reclamation.

Dr. Kent also investigates other parasites, including those infecting terrestrial animals, and is exploring the taxonomic variability of Neorickettsia spp. associated with salmon poisoning, a common and deadly disease of dogs in Oregon.  This work is in collaboration with Dr. Sanders and Dr. Vasyl Tkach (University of North Dakota).

AWARDS IN 2018

With appointments in the College of Science, College of Agriculture, and the Carlson College of Veterinary Medicine, Dr. Kent received both the OSU Alumni Association Distinguished Professor Award and the Agricultural Research Foundation Distinguished Faculty Award for his superior academic performance, professional renown and service to the University and to the Public.  He also received the 2018 Outstanding Steward of Zebrafish Husbandry from the Zebrafish Husbandry Association (International Society) for his research with zebrafish.

See-through Casper zebrafish. Green - GFP labeled Mycobacterium marinum in various organs.  Red=heart (this is a "red heart mutant").

Casper (transparent) zebrafish infected with fluorescently-labeled Toxoplasma gondii (arrow) Pseudocapillaria tomentosa.
A nematode in the intestine
of a zebrafish.
Contracaecum larval worm in sucker heart (left).  Worm completely replaces ventricle.  Normal heart on right. Photo:  A. Janik, OSU.

SELECTED PUBLICATIONS

Pub Med

Gaulke, C.A., Martins, M.L., Watral, V.G., Humphreys, I.R., Spagnoli, S.T., Kent, M.L., and Sharpton, T.J.  2019.  A longitudinal assessment of host-microbe-parasite interactions resolves the zebrafish gut microbiome's link to Pseudocapillaria tomentosa infection and pathology.  Microbiome. PMID 30678738.

Kent, M.L., Gaulke, G.A., Watral, V., and Sharpton, T.J.  2018.  Pseudocapillaria tomentosa in laboratory zebrafish (Danio rerio):  Patterns of infection and dose response.  Dis. Aquat. Org. (in press).

Colvin, M.E., Peterson, J.T., Sharpe, C., Kent, M.L., and Schreck, C.B.  2018.  Identifying optimal hauling densities for adult run Chinook salmon trap and haul operations.  River Res. Appl.  (in press).

Murray, K.N., Wolf, J.C., Spagnoli, S.T., Lains, D., Budrow, N., and Kent, M.L.  2018.  Reversibility of proliferative thyroid lesions induced by iodine deficiency in a laboratory zebrafish colony.  Zebrafish doi:10.1089/zeb.2018.1603.

Janik, A.J., Markle, D.F., Heidel, J.R., and Kent, M.L.  2018.  Histopathology and external examination of heavily parasitized Lost River Sucker Deltistes luxatus (Cope 1879) and Shortnose Sucker Chasmistes brevirostris (Cope 1879) from Upper Klamath Lake, Oregon.  J. Fish Dis. doi:10.1111/jfd.12875.

Herron, C.L., Kent, M.L. and Schreck, C.B.  2018.  Swimming endurance in juvenile chinook salmon infected with Salmincola californiensis.  J. Aquat. Anim. Health 30(1):81-89.

Norris, L.J., Watral, V., and Kent, M.L.  2018.  Survival of bacterial and parasitic pathogens from zebrafish (Danio rerio) after cryopreservation and thawing.   Zebrafish 15(2):188-201.

Martins, M.L, Tavares-Dias, M., Janik, A.J., Kent, M.L., and Jerônimo, G.T.  2017.  Hematology and condition factor of tui chub and fathead minnow parasitized by nematode from Upper Klamath Lake, Oregon, USA.  Dis. Aquyat. Organ. 126(3):257-262.  

Burns, A.R., Watral, V., Sichel, S., Spagnoli, S., Banse, A.V., Mittge, E., Sharpton, T.J., Guillemin, K., and Kent, M.L.  2018. Transmission of a common intestinal neoplasm in zebrafish by cohabitation. J Fish Dis. Oct 11. doi: 10.1111/jfd.12743. [Epub ahead of print]. 

Wilson, A.K., Watral, V.G., Kent, M.L., Sharpton, T.J., and Gaulke, C.A.  2017. Draft Genome Sequence of Pseudomonas sp. Strain DrBHI1 (Phylum Proteobacteria).  Genome Announc. Sep 28;5(39).  PMID: 29023774

Meritet, D.M., Mulrooney, D.M., Kent, M.L. and Lohr, C.V.  2017.  Development of quantitative real-time PCR assays for postmortem detection of Mycobacterium spp. common in zebrafish (Danio rerio) research colonies.  J. Am. Assoc. Lab Animal Sci. 56(2):131-141.

Greiman, S.E., Kent, M.L., Betts, J., Cochell, D., Sigler, T., and Tkach, V.V.  2016.  Nanophyetus salmincola, vctor of the salmon poisoning disease agent Neorickettsia helminthoeca, harbors a second pathogenic Neorickettsia species.  Vet. Parasitol.  299:107-109.

Kent, M.L., Hsia, H.J., Van de Ven, T.J. and Buchheit, T.E.  2016.  Perioperative pain management strategies for amputation:  A topical review.  Pain Med.  pii: pnwl 10; PMID 271402960.

Spagnoli, S., Sanders, J. and Kent, M.L.  2016.  The common neural parasite Pseudoloma neurophilia causes altered shoaling behaviour in adult laboratory zebrafish (Danio rerio) and its implications for neurobehavioural research.  J Fish Dis. 2016 Jul 11. doi: 10.1111/jfd.12512. [Epub ahead of print] PMID:  27396581.

Stentiford, G.D., Becnel, J.J., Weiss, L.M., Keeling, P.J., Didier, E.S., Williams, B.A., Bjornson, S., Kent, M.L., Freeman, M.A., Brown, M.J., Troemel, E.R., Roesel, K., Sokolova, Y., Snowden, K.F., and Solter, L.  2016.  Microsporidia - Emergent Pathogens in the Global Food Chain. Trends Parasitol. 32(4):336-48.

Mason, T., Snell, K., Mittge, E., Melancon E., Montgomery, R., McFadden, M., Camorlano, J., Kent, M.L., Whipps, C.M., and Peirce, J.  2016.  Strategies to mitigate a Mycobacterium marinum outbreak in a zebrafish research facility.  Zebrafish PMID 27159037.

Sanders, J.L., Watral, V., Stidworthy, M.F., and Kent, M.L.  2016.  Expansion of the known host range of the microsporidium, Pseudoloma neurophilia.  Zebrafish PMID 27182659.

Spagnoli, S., Sanders, J. Watral, V. and Kent, M.L.  2016.  Pseudoloma neurophilia infection combined with gamma irradiation causes increased mortality in adult zebrafish (Danio rerio) compared to infection or irradiation alone:  New implications for studies involving immunosuppression.  Zebrafish PMID 27123755.

Murray, K.N., Varga, Z.M., and Kent, M.L.  2016.  Biosecurity and health monitoring at the zebrafish international resource center.  Zebrafish PMID 27031282.

Kent, M.L., Watral, V.G., Kirchoff, N.S., Spagnoli, S.T. and Sharpton, T.J.  2016.  Effects of subclinical Mycobacterium chelonae infections on fecundity and embryo survival in zebrafish.  Zebrafish PMID 27031171.

Stentiford, G.D., Becnel, J.J., Weiss, L.M., Keeling, P.J., Didier, E.S., Williams, B.A., Bjornson, S., Kent, M.L., Freeman, M.A., Brown, M.J., Troemel, E.R., Roesel, K., Sokolova, Y., Snowden, K.F. and Solter, L.  2016.  Microsporidia - Emergent Pathogens in the Global Food Chain.  Trends Parasitol.  pii:S1471-4922.

Dolan, B.P., Fisher, K.M., Colvin, M.E., Benda, S.E., Peterson, J.T., Kent, M.L. and Schreck, C.B.  2016.  Innate and adaptive immune responses in migrating spring-run adult chinook salmon, Oncorhynchus tshawytscha.  Fish Shellfish Immunol. 48:136-44.

Clemens, B.J., Spangler, J., Robertson, P., Gaolvich, G., Banner, G., Avery, D., Gunckel, S., Bowdem, K., Ketchum, L., Spagnoli, S., Kent, M., and Kirk, J.P.  In press.  Evidence suggesting that grass carp of Devils Lake, Oregon, are the oldest in North America.  N. Am. J. Fish. Mgmt.

Dolan, B.P., Fisher, K.M., Colvin, M.E., Benda, S.E., Peterson, J.T., Kent, M.L., and Schreck, C.B.  2015.  Innate and adaptive immune responses in mirgrating spring-run adult chinook salmon, Oncorhynchus tshawytscha.  Fish Shellfish Immunol.  S1050-4648(15)30234-5. 

Benda, S.E., Kent, M.L., Caudill, C.C., Schreck, C.B., and Naughton, G.  2015.  Cool, pathogen free refuge lowers pathogen associated prespawn mortality of Willamette River Chinook Salmon (Oncorhynchus tshawytscha).  Trans. Am. Fish Soc. 144(6):1159-1172.

Paquette, C.E., Kent, M.L., Peterson, T.S., Wang, R., Dashwood, R.H., and Löhr, C.V.  2015.  Immunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin.  Dis. Aquat. Organ. 116(3):191-7.

Uhrig, E.J., Spagnoli, S.T., Tkach, V.V., Kent, M.L. and Mason, R.T.  2015.  Alaria mesocercariae in the tails of red-sided garter snakes:  evidence for parasite-mediated caudectomy.  Parasitol. Res. 114(12):4451-61.

Sanders, J.L., Jaramillo, A.G., Ashford, J.E., Feist, S.W., Lafferty, K.D. and Kent, M.L.  2015.  Two Myxozoans from the urinary tract of topsmelt, Atherinops affinis.  J. Parasitol. 101(5):577-86.

Spagnoli, S., Xue, L. and Kent, M.L.  2015.  The common neural parasite Pseudoloma neurophilia is associated with altered startle response habituation in adult zebrafish (Danio rerio):  Implications for the zebrafish as a model organism.  Behav. Brain Res.  doi:10.1016/j.bbr.2015.05.046.

Sanders, J.L., Zhou, Y., Moulton, H.M., Moulton, Z.X., McLeod, R.,  Dubey, J.P., Weiss, L.M., and Kent, M.L.  2015.  The zebrafish, Danio rerio, as a model for Toxoplasma gondii:  an initial description of infection in fish.  J. Fish. Dis.  38(7):675-9.

Spagnoli, S.T., Xue, L., Murray, K.N., Chow, F., and Kent, M.L.  2015.  Pseudoloma neurophilia:  a retrospective and descriptive study of nervous system and muscle infections, with new implications for pathogenesis and behavioral phenotypes.  Zebrafish.  12(2):189-201.

Feist, S.W., Stentiford, G.D., Kent, M.L., Ribeiro-Santos, A., and Lorance, P.  2015.  Histopathological assessment of liver and gonad pathology in continental slope fish from the northeast Atlantic Ocean.  2015.  Mar. Environ. Res. 106:42-50.

Colvin, M.E., Peterson, J.T., Kent, M.L., and Schreck, C.B.  2015.  Occupancy modeling for improved accuracy and understanding of pathogen prevalence and dynamics.  PLoS One. 10(3):e0116605.

Chow, F.W., Xue, L. and Kent, M.L.  2015.  Retrospective study of the prevalence of Pseudoloma neurophilia shows male sex bias in zebrafish Danio rerio (Hamilton-Buchanan).  J.Fish Dis. PMID:  25588311.

Whipps, C.M., Kent, M.L. and Murray, K.N.  2014.  Occurrence of a myxozoan parasite Myxidium streisingeri N. sp. in laboratory zebrafish Danio rerio.  2014.  J. Parasitol.  PMID:  25277837.

Hooper, K.J.  Bobe, G., Vorachek, W.R., Bishop-Stewart, J.K., Mosher, W.D., Pirelli, G.J., Kent, M.L., Hall, J.A.  Effect of selenium yeast supplementation on naturally acquired parasitic infection in ewes.  Biol. Trace Elem. Res. 161(3):308-17.

Lafferty, K.D., Harvell, C.D., Conrad, J.M., Friedman, C.S., Kent, M.L., Kuris, A.M., Powell, E.N., Rondeau, D., and Saksida, S.M.  2014.  Infectious diseases affect marine fisheries and aquaculture economics.  Ann. Rev. Mar. Sci. PMID 25251276.

Collymore, C., Watral, V., White, J.R., Colvin, M.E., Rasmussen, S., Tolwani, R.J. and Kent, M.L.  Tolerance and efficacy of emamectin benzoate and ivermectin for the treatment of Pseudocapillaria tomentosa in laboratory zebrafish (Danio rerio).  Zebrafish 11(5):490-7.

Wolf, J.C., Baumgartner, W.A., Blazer, V.S., Camus, A.C., Engelhardt, J.A., Fournie, J.W., Frasca, S., Jr., Groman, D.B., Kent, M.L., Khoo, L.H., Law, J.M., Lombardini, E.D., Ruehl-Fehlert, C., Segner, H.E., Smith, S.A., Spitsbergen, J.M>, Weber, K., Wolfe, M.J.  2014.  Toxicol. Pathol. PMID:  25112278.

Kent, M.L., Soderlund, K., Thomann, E., Schreck, C.B., and Sharpton, T.  2014.  Post-mortem sporulation of Ceratomyxa shasta (myxozoa) after death in adult chinook salmon.  J. Parasitol.  PMID 247250089.

West, K., Miles, R., Kent, M.L., and Frazer, J.K.  2014.  Unusual fluorescent granulomas and myonecrosis in Danio rerio infected by the microsporidian pathogen Pseudoloma neurophilia.  Zebrafish. PMID 24707848.

Kent, M.L., Buchner, C., Barton, C., and Tanguay, R.L.  2014.  Toxicity of chlorine to zebrafish embryos.  Dis. Aquat. Organ.  107(3):235-40. 

Sanders, J.L.  Peterson, T.S., and Kent, M.L.  2014.  Early development and tissue distribution of Pseudoloma neurophilia in the zebrafish, Danio rerio.  J. Eukaryot. Microbiol. 61(3):238-64.

Sanders, J.L., Kent, M.L.  2013.  Verification of intraovum transmission of a microsporidium of vertebrates:  Pseudoloma neurophilia infecting the zebrafish, Danio rerio.  PLOS one.  8(9):e76064.DOI:10.1371/journal.pone.0076064.

Peterson, T.S., Ferguson, J.A., Watral, V.G., Mutoji, K.N., Ennis, D.G., Kent, M.L.  2013.  Paramecium caudatum enhances transmission and infectivity of Mycobacterium marinum and Mycobacterium chelonae in zebrafish (Danio rerio).  Dis. Aquat. Org. 106:229-239.

Kent, M.L., Benda, S., St.-Hillaire, S., Schreck, C.B.  2013.  Sensitivity and specificity of histology for diagnoses of four common pathogens and detection of nontarget pathogens in adult Chinook salmon (Oncorhynchus tshawytscha) in freshwater.  J. Vet. Diag. Invest., 25:341-351.

Neal, J.T., Peterson, T.S, Kent, M.L., Guillemin, K.  2013.  H. pylori virulence factor CagA increases intestinal cell proliferation by Wnt pathway activation in a transgenic zebrafish model.  Dis. Model Mech. Mar. 1.

Kent, M.L., Harper, C., Wolf, J.C.  2012.  Documented and potential research impacts of subclinical diseases in zebrafish.  ILAR J.  53:  126-134.

Sanders, J.S., Kent, M.L.  2012.  Control of microsporidiosis in laboratory zebrafish colonies.  ILAR J.  53:  106-113.

Sanders, J., Myers, M.S., Tomanek, L., Cali, A M., Takvorian, P,M.  Kent, M.L. 2012.  Ichthyosporidium weissii n. sp. (Microsporidia) infecting the arrow goby (Clevelandia ios).  J. Eurk. Microbiol. (J59:  258-267).

Ferguson, J.A., Koketsu, W.,  Ninomiya, I.,  Rossignol,  P.A.,  Jacobson, K.C., Kent, M.L. 2011.  Mortality of coho salmon (Oncorhynchus kisutch) associated with burdens of multiple parasite species.  Intl. J. Parasitol. 41: 1197-11205.

Sanders J.L., Kent M.L.   2011. Development of a sensitive assay for the detection of Pseudoloma neurophilia in laboratory ­populations of the zebrafish Danio rerio. Dis Aquat Org. 96:145-156

Ferguson, J.A., Romer, J., Sifneos, J., Madsen, L, Glynn, M., Schreck, C.B., Kent, M.L.  2012. Impacts of multispecies parasitism on juvenile Oregon Coho Salmon (Oncorhynchus kisutch).  Aquaculture  (in press, available online).

Ferguson, J.A., St-Hilaire, S., Peterson, T.S., Rodnick, K., Kent, M.L. 2011. Survey of parasites in threatened stocks of coho salmon (Oncorhynchus kisutch) in Oregon by examination of wet tissues and histology.  J. Parasitol.  97: 1085-1098.

Peterson, T.S., Spitsbergen, J.M., Feist, S.W., Kent, M.L. 2011. The Luna stain, an improved selective stain for detection of microsporidian spores in histologic sections. Dis. Aquat. Org.  95: 175–180.

Kent, M.L., Buchner, C., Watral, V.G., Sanders, J.L. LaDu, J., Peterson, T.S., Tanguay, R.L. 2011. Development and maintenance of a specific pathogen free (SPF) zebrafish research facility for Pseudoloma neurophilia.  Dis. Aquat. Org. 95: 73–79.

Ferguson, J.A., Woodberry, K., Gillin, C.M., Jackson, D.H., Sanders, J., Madigan, W., J. Bildfell, R.J., Kent, M.L. 2011. Cylicospirura species (Nematoda: Spirocercidae) and stomach nodules in cougars (Puma concolor) and bobcats (Lynx rufus) in Oregon. J. Wildl. Dis. 47:140-153.

Brown, A. M. V., Kent, M.L., Adamson, M.L. 2010. Description of five new Loma (Microsporidia) species in Pacific Fishes with redesignation of the type species Loma morhua Morrison & Sprague, 1981, based on morphological and molecular species-boundaries tests. J. Eurk. Microbiol. 57: 529-553.

Jurasek, M.E. Bishop-Stewart, J.K., Storey, B.E., Kaplan, R.M., Kent, M.L.  2010. Modification and further evaluation of a fluorescein-labeled peanut agglutinin test for identification of Haemonchus contortus eggs. Vet. Parasitol.  69:209-213.

Sanders, J.L., Lawrence, C., Nichols, D.K., Bru , J.F., Peterson, T.S., Murray, K.N., Kent, M.L. 2010. Pleistophora hyphessobryconis (Microsporidia) infecting zebrafish 9911:47-56.

Brown, A.M.V., Kent, M.L., Adamson, M.L. 2010.  Low genetic variation in the salmon and trout parasite Loma salmonae (Microsporidia) supports marine transmission and clarifies species boundaries. Dis. Aquat. Org. 91:35-46.

Mansfield, K.G., Riley, L., Kent, M.L.  2010. Workshop summary: detection, impact, and control of specific pathogens in animal resource facilities. ILAR. 51:171-179.

Whipps, C.M., Boorom, K., Bermudez, L.E., Kent, M.L. 2010. Molecular characterization  of Blastocystis species in Oregon identifies multiple subtypes. Parasitol. Res. 106: 827-832.

Landers, D.H. , Simmonich, S.M., Jaffe, D., Geiser, L., Campbell, D.H., Schwindt, A.,  Schreck, C.B., Kent, M.L., Hafner, W., Kimberly, H., Usenko, S., Ackerman, L., Schrlau, J., Rose, N., Blett, T.,  Erway, M. M. 2010. The Western Airborne Contaminant Assessment Project (WACAP): An Interdisciplinary Evaluation of the Impacts of Airborne Contaminants in Western U. S. National Parks. Environ. Sci. Tech. Environ.44: 855–859.

Ramsay, J.M., Watral, V., Schreck, C.B., Kent, M.L. 2009. Husbandry stress exacerbates mycobacterial infections in adult  zebrafish, Danio rerio (Hamilton). J. Fish Dis. 32: 931-941.

Schwindt, A.R., Kent, M.L., Ackerman, L.K., Simonich, S.L., Landers, D.H., Blett, Schreck, C.B. 2009. Reproductive abnormalities in fish from Western U.S. National Parks. Trans. Am. Fish Soc. 138: 522-531.

Jacobs, J.M., Stine, C.S., Baya, A.M., Kent, M.L. 2009. A review of mycobacteriosis in marine fishes. J. Fish Dis. 32: 119-130.

Selected Book Chapters

Saksida, S.M., Gardner, I., and Kent, M.L.  2014.  Transmission of infectious agents between wild and farmed fish.  Chapter 9.  IN:  Woo, P.T.K. and Bruno, D.  Diseases of Finfish in Cage Culture.  CAB Intl. Publ.  Wallingford, Oxon, England, pp. 313-329.

Kent, M.L., Shaw, R.W., and Sanders, J.  2014.  Fish Microsporidia.  Chapter 20.  IN:  Microsporidia:  Pathogens of Opportunity.  IN:  Weiss, L.M. and Becnel, J.J.  (eds.).  Wiley Enterprise.  pp. 357-370.

Kent, M.L. and Fournie, J.  2007.  Parasites of Fishes.  IN:  D.G.  Baker (eds.).  Flynn's Parasites of Laboratory Animals.  Blackwell Publ.  pp. 69-117.